Acute Effects of Moderate-Intensity Exercise on Plasma Free Fatty Acid Levels in Normal-Weight Women
Downloads
Objective: Physical activity supports metabolic health by enhancing energy metabolism, including lipolysis—the breakdown of fat stores into glycerol and free fatty acids. However, the impact of moderate-intensity ergocycle and circuit body weight training (CBWT) on FFA concentrations in adolescent women remains unclear. This study aimed to assess moderate-intensity ergocycle training and CBWT against free fatty acid concentrations on the process of lipolysis.
Methods and Materials: This study was to quasi experiment pretest-posttest group design. The subject of this study whose the 26 women aged 18- 23 years, divided into two groups randomly so that each group made up of 13 people. The first group (K1) was an ergocycle training and the second group (K2) was an exercise CBWT. Blood sampling was performed at baseline (pre) and 5 minutes post-exercise. ELISA was applied to evaluate the concentration of free fatty acids in all samples. Statistical analysis was applied using paired sample t-test and independent sample t-test with a significance level of 5%.
Findings: Resultsn showed that the concentration of free fatty acids in K1 between pre and post (0.054±0.002 to 0.057±0.004 mmol/L, p=0.012, effect size (ES): 0.949). In K2 (0.056±0.003 to 0.065±0.007 mmol/L, p=0.001, ES: 1.671). We also observed differences between groups (p < 0.001, ES: 1.403).
Conclusion: Both exercise models have been shown to improve free fatty acid concentrations. However, the CBWT model was found to be more effective than ergocycle in improving free fatty acid concentrations in normal-weight women.
Downloads
Archila, L. R., Bostad, W., Joyner, M. J., & Gibala, M. J. (2021). Simple bodyweight training improves cardiorespiratory fitness with minimal time commitment: a contemporary application of the 5BX approach. International journal of exercise science, 14(3), 93. https://doi.org/10.70252/WEQD2681.
Arner, P. (2005). Human fat cell lipolysis: biochemistry, regulation and clinical role. Best practice & research Clinical endocrinology & metabolism, 19(4), 471-482. https://doi.org/10.1016/j.beem.2005.07.004.
Christensen, R. H., Wedell-Neergaard, A.-S., Lehrskov, L. L., Legaard, G. E., Dorph, E., Larsen, M. K., Launbo, N., Fagerlind, S. R., Seide, S. K., & Nymand, S. (2019). Effect of aerobic and resistance exercise on cardiac adipose tissues: secondary analyses from a randomized clinical trial. JAMA cardiology, 4(8), 778-787. https://doi.org/10.1001/jamacardio.2019.2074
Despres, J., Bouchard, C., Savard, R., Tremblay, A., Marcotte, M., & Theriault, G. (1984). The effect of a 20-week endurance training program on adipose-tissue morphology and lipolysis in men and women. Metabolism, 33(3), 235-239. https://doi.org/10.1016/0026-0495(84)90043-x.
Dyaksa, R. S., Liben, P., Mintarto, E., Herawati, L., & Al-Arif, M. A. (2021). Low and Moderate Intensity Exercise Decreased Body Fat and Increased Free Fatty Acid in Overweight Women. Folia Medica Indonesiana, 57(4), 272-276. https://doi.org/10.20473/fmi.v57i4.11473.
Farsi, S., & Ghahramani, M. (2020). Molecular adaptations of lipolysis to physical activity. Journal of Basic Research in Medical Sciences, 7(1), 1-9. https://jbrms.medilam.ac.ir/browse.php?a_id=450&sid=1&slc_lang=fa
Gemmink, A., Schrauwen, P., & Hesselink, M. K. (2020). Exercising your fat (metabolism) into shape: a muscle-centred view. Diabetologia, 63(8), 1453-1463. https://doi.org/10.1007/s00125-020-05170-z.
Göger, S., & Cingil, D. (2022). Healthy lifestyle behaviors among 18-to 49-year-old women: a comparative study. Community Health Equity Research & Policy, 42(3), 239-244. https://doi.org/10.1177/0272684X20973833.
Henderson, G. C. (2021). Plasma free fatty acid concentration as a modifiable risk factor for metabolic disease. Nutrients, 13(8), 2590. https://doi.org/10.3390/nu13082590.
Holloszy, J. O., & Coyle, E. F. (1984). Adaptations of skeletal muscle to endurance exercise and their metabolic consequences. Journal of applied physiology, 56(4), 831-838. https://doi.org/10.1152/jappl.1984.56.4.831.
Kim, J.-W., Ko, Y.-C., Seo, T.-B., & Kim, Y.-P. (2018). Effect of circuit training on body composition, physical fitness, and metabolic syndrome risk factors in obese female college students. Journal of exercise rehabilitation, 14(3), 460. https://doi.org/10.12965/jer.1836194.097.
Kumar, D., & Dhull, K. N. S. (2023). A comprehensive analysis of circuit training: Assessing the benefits and drawbacks for diverse fitness goals. Journal of Sports Science and Nutrition, 4(1), 190-193. https://doi.org/10.33545/27077012.2023.v4.i1c.194.
Lafortuna, C. L., Agosti, F., Galli, R., Busti, C., Lazzer, S., & Sartorio, A. (2008). The energetic and cardiovascular response to treadmill walking and cycle ergometer exercise in obese women. European journal of applied physiology, 103(6), 707-717. https://doi.org/10.1007/s00421-008-0758-y.
McMurray, R. G., & Hackney, A. C. (2005). Interactions of metabolic hormones, adipose tissue and exercise. Sports medicine, 35(5), 393-412. https://doi.org/10.2165/00007256-200535050-00003.
Myers, J., Kokkinos, P., & Nyelin, E. (2019). Physical activity, cardiorespiratory fitness, and the metabolic syndrome. Nutrients, 11(7), 1652. https://doi.org/10.3390/nu11071652.
Paoli, A., Casolo, A., Saoncella, M., Bertaggia, C., Fantin, M., Bianco, A., Marcolin, G., & Moro, T. (2021). Effect of an endurance and strength mixed circuit training on regional fat thickness: the quest for the “spot reduction”. International journal of environmental research and public health, 18(7), 3845. https://doi.org/10.3390/ijerph18073845.
Petridou, A., Nikolaidis, M. G., Matsakas, A., Schulz, T., Michna, H., & Mougios, V. (2005). Effect of exercise training on the fatty acid composition of lipid classes in rat liver, skeletal muscle, and adipose tissue. European journal of applied physiology, 94(1), 84-92. https://doi.org/10.1007/s00421-004-1294-z.
Rezaei, M., Taheri, M., Irandoust, K., & Bragazzi, N. L. (2024). The Effects of a Period of Aerobic Exercises on Sexual Hormones and Appetite in Obese Women with Polycystic Ovary Syndrome. International Journal of Body, Mind & Culture (2345-5802), 11(4). https://doi.org/10.22122/ijbmc.v11i4.770
Risikesan, J., Heebøll, S., Kumarathas, I., Funck, K. L., Søndergaard, E., Johansen, R. F., Ringgaard, S., Tolbod, L. P., Johannsen, M., & Kanstrup, H. L. (2023). Exercise increases myocardial free fatty acid oxidation in subjects with metabolic dysfunction-associated fatty liver disease. Atherosclerosis, 372, 10-18. https://doi.org/10.1016/j.atherosclerosis.2023.03.015.
Tremblay, A., Després, J.-P., & Bouchard, C. (1985). The effects of exercise-training on energy balance and adipose tissue morphology and metabolism. Sports medicine, 2(3), 223-233. https://doi.org/10.2165/00007256-198502030-00005
Copyright (c) 2026 International Journal of Body, Mind and Culture

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.








